Growth hormone releasing hormone (GRH) kit
Introduction
What is the growth hormone releasing hormone tumor? Clinically, somatostatin is a 14 amino acid polypeptide originally isolated from the rat hypothalamus in 1968 and found to inhibit the release of growth hormone. It is named as a growth hormone release inhibitor. Thalamic pancreatic D cells have somatostatin in the gastroduodenum and small intestine and can widely inhibit the release of various peptides. Therefore, this hormone can not only inhibit endocrine and exocrine but also inhibit intestinal peristalsis and gallbladder contraction. In order to inhibit hormones, Ganda and Larsson first independently described somatostatin tumors in their own reports in 1977.
Growth hormone releasing hormone (GRH) kit
Experimental condition selection
In ELISA, it is important to select the various experimental conditions, including:
(1) Selection of solid phase support: Many substances can be used as solid phase carriers such as polyvinyl chloride, polystyrene, polyacrylamide and cellulose. The form may be a flat plate, a test tube, a bead or the like. Currently used is a 40-well polystyrene recessed plate. Regardless of the carrier, screening can be carried out before use: the reaction is carried out under the same experimental conditions by coating with an equal amount of antigen, and whether the coloration reaction is uniform or not, and whether the adsorption performance is good.
(2) Selection of coated antibody (or antigen): When the antibody (or antigen) is adsorbed on the surface of the solid phase carrier, the purity is required to be good, and the pH is generally required to be between 9.0 and 9.6. The adsorption temperature, time and the amount of protein also have a certain influence, and generally use 4 ° C for 18 to 24 hours. The optimum concentration of protein coating is titrated: after coating with different protein concentrations (0.1, 1.0, and 10 μg/ml, etc.), the OD value of the positive specimen is observed when the other test conditions are the same. The concentration with the highest OD value and the least amount of protein is selected. It is usually 1 to 10 μg/ml for most proteins.
(3) Selection of working concentration of enzyme-labeled antibody: First, titration of preliminary titer is carried out by direct ELISA (see enzyme-labeled antibody fraction). Then, other conditions are fixed or the "square matrix method" (the coating, the reference sample of the sample to be tested, and the enzyme-labeled antibody are respectively different dilutions) are accurately titrated in the formal experimental system.
(4) Enzyme substrate and hydrogen donor selection: The choice of hydrogen donor is cheap, safe, and has obvious color reaction, but it is colorless. Some hydrogen donors (such as OPD) have potential carcinogenic effects and should be protected. Those who are qualified should use hydrogen donors that are not carcinogenic and sensitive. For example, TMB and ABTS are currently satisfactory hydrogen donors. After the substrate has been applied for a while, a strong acid or a strong base should be added to terminate the reaction. Usually the substrate action time is preferably 10-30 minutes. The substrate used must be freshly prepared, especially H2O2 before use.
The elisa kit sold by our company has high detection sensitivity, good specificity and stable quality. Purchase kits, enjoy the authority of enzyme-linked immunoassay technology services, provide free professional agency services, enjoy zero-cost transportation, and improve after-sales. ELISA kit, quality assurance! Provide technical guidance throughout the sale before sale, quality problems can be returned free of charge!
Many customers have various concerns and concerns during the consultation and comparison kits. Xinfan Bio is committed to giving you quality assurance, exempting you from worry, and letting you buy the rest assured and comfortable.
Purchase the company's elisa kit, enjoy zero-cost shipping, and improve after-sales. In addition, you can enjoy the authoritative enzyme-linked immunoassay service, skilled operators, and sophisticated laboratory equipment to ensure that each experimental data is true and reliable. The ELISA kits sold by the company are of quality assurance! Technical guidance is provided throughout the sale and after sale, and there are quality problems, which can be returned free of charge!
Newmax Electronics Co.,Limited , https://www.advvape.com