Jinma teaches you how to deal with ELISA kit results judgment - Database & Sql Blog Articles

EL-C1600N100013-B

WIFI test head can be customized for other specifications

Test - lowercase jpg

Kaixin micro test

Judging the results of the elisa kit experiment is the last key step. In this session, the teachers are most concerned about it, but there are often some problems in the experiment results. In response to the Elisa results, Shanghai Jinma will give you some ideas:

First, Elisa results to determine common problems

1. Including negative control wells, the color development of each well is generally deep;

2. There is no coloration in each well including the positive control;

3. The positive control did not develop color, while the other samples showed normal color;

4. Some specimens are not dark in color and it is difficult to judge positive.

Second, the solution

1. It may be insufficient washing; excessive loading; excessive incubation time is too high; follow the instructions for re-operation. If there is no improvement, you can contact the manufacturer;

2. It may be that the sample or enzyme solution is missing; the incubation time is too short and the temperature is too low; the activity of the reagent is not properly preserved; follow the instructions. If there is no improvement, you can contact the manufacturer;

3. The positive control misses, adds or fails. Re-operate. If there is no improvement, you can contact the manufacturer and ask for the reference product;

4. Re-operate. It is best to use a microplate reader to measure the light absorbance and judge according to the P/N value standard.
Shanghai Jinma provides you with a full range of services. You can call us if you encounter any problems during the experiment. Our website regularly updates technical articles and welcomes attention.