Mouse total complement (CH50) ELISA kit method - Database & Sql Blog Articles

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EL-C1600N100013-B
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Mouse total complement (CH50) elisa kit use method

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1. Serum: Avoid any cellular irritation during the procedure. Use tubes without pyrogens and endotoxins. After collecting the blood, the red blood cells were quickly and carefully separated by centrifugation at 1000 x g for 10 minutes.
2. Plasma: EDTA, citrate, heparin plasma can be used for detection. The pellet was removed by centrifugation at 1000 x g for 30 minutes.
3. Cell supernatant: Centrifuge at 1000 xg for 10 minutes to remove particles and polymer.
4. Tissue homogenization: the tissue is added to the appropriate amount of physiological saline and mashed. Centrifuge at 1000 xg for 10 minutes and take the supernatant.
5. Storage: If the sample is not used immediately, it should be divided into small parts - 70 ° C to avoid repeated freezing. Do not use hemolysis or hyperlipemia as much as possible. If there are large amounts of particles in the serum, centrifuge or filter before testing. Do not thaw at 37 ° C or higher. Thaw at room temperature and ensure that the sample is fully thawed.

Mouse total complement (CH50) ELISA kit is safe to use:
1. Avoid direct contact with the stop solution and substrate. If you come into contact with these liquids, rinse them off with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not use your mouth to take any ingredients from the kit.

Mouse total complement (CH50) ELISA kit (company provides free testing services and technical guidance)

Product Name: Mouse50%complementhemolysis, CH50 ELISA Kit

Product specifications: 48T/96T
Product Categories:

Mouse ELISA kit


Storage conditions: 2-8 ° C

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