In the ELISA kit experiment, why should serum test and serum test affect the experimental results? -Huaqiang Electric

In fact, ELISA can measure some tissues, cells, etc., but because the tissue and cells are solid samples, they are broken and extracted, and the substances to be tested are extracted (such as mechanical crushing, ultrasonic disruption, There are differences in the components of the lysate, etc., and the composition of the extract, which eventually leads to a difference in the degree of extraction, making it difficult to establish a normal reference value; while serum or plasma samples do not have deviations due to the extraction process and are very convenient to collect. Therefore, serum and plasma are often used as test subjects [kit]. Note: 1. When mixing protein solution, try to be gentle and avoid foaming. 2. The washing process is very important, and insufficient washing can cause false positives. 3. It is best to control the loading time within 5 minutes. If the number of specimens is large, it is recommended to use a gun. 4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. 5. If the content of the substance to be tested in the specimen is too high, please dilute it before measuring it. When calculating, multiply by the dilution factor. 6. When preparing the standard and test solution working solution, please prepare with the corresponding dilution solution, not to be confused. 7. Keep the substrate away from light. 8. Do not replace the reagents in the kit with reagents from other manufacturers. Jianglai Biotech provides a variety of ELISA scientific test kits for various species and various series. Buy any of the company's elisa kits, can provide generation of testing services, direct sales, quality is guaranteed, the model can be shipped the same day, free express delivery, details can consult our company customer service