Diamine oxidase (DAO) distribution is widely distributed in animal tissues (intestinal mucosa, lung, liver, kidney, etc.), plant tissues and microorganisms. The enzyme in animal tissues is different from monoamine oxidase and is water-soluble. It is present in the homogenate supernatant and contains the coenzyme pyridoxal in the enzyme of the relatively refined kidney. There may also be FAD or copper. Although the Michaelis constant is large, it can also oxidize monoamine.
Diamine oxidase (DAO) inhibits enzyme activity and is inhibited by carbonyl reagents such as hydroxylamine and semicarbazide.
Diamine oxidase (DAO) summarizes that the enzyme can decompose histamine, and can also decompose the amine formed by decarboxylation of amino acids in the intestinal mucosa, which plays a detoxifying role. It is crystallized from ruthenium in ruminant plasma, which oxidizes benzylamine, spermine, and has a molecular weight of 250,000, containing copper and pyridoxal phosphate. Plant enzymes also oxidize monoamines well, containing copper, in pink. Microbial enzymes are also highly specific to the matrix and can be induced to form.
Shanghai Xinfan provides human diamine oxidase (DAO) ELISA kit, available from stock, provides technical guidance throughout the process, provides after-sales service and free agent testing services, welcome to inquire.
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